Abstract: Objective The aim is to investigate the difference of gene expression between MEF-regDC and imDC & maDC, in order to demonstrate the character of MEF-regDC. Methods We first harvest MEF-regDC by co-culture of bone marrow hematopoietic stem cell with MSC, then analysis 3 types of DCs, including Fold change, Principal Component Analysis and GoTerm to Gene. Results MEF-regDC has the characteristic of morphology, phenotypes and gene expression profile, the PCA illustrate that MEF-regDC and imDC & maDC are 3 different type of DC, and there are 13369 genes that are 2 folds different between MEF-regDC and imDC, and the number of genes that are 6 folds different between the 2 types of DCs is 3247; meanwhile, the number of different genes between MEF-regDC and maDC are 11326 and 3299, respectively. Further analysis demonstrate that, there are obvious difference of function regulate genes, immune-related genes and signaling pathways related genes among these three type of DCs. Conclusion MEF-regDC is a new type of DC which is different from imDC and maDC, and has characteristic gene expression profiles.

Key words: dendritic cells, mesenchymal stem cells, gene differentiation, gene analysis