Basic & Clinical Medicine ›› 2014, Vol. 34 ›› Issue (11): 1491-1496.

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Effects of β-catenin on cell migration、invasion and apoptosis of TE85 osteosarcoma cells


  • Received:2014-06-20 Revised:2014-09-25 Online:2014-11-05 Published:2014-11-03

Abstract: Objective To investigate the effect of β-catenin on the migration, invasion and apoptosis of osteosarcoma cell line TE85. Methods The mRNA and protein expression of total β-catenin in different osteosarcoma cell lines were detected by RT-PCR and Western blot, and the osteosarcoma cell line TE85 was infected with Adβ-catenin, then RT-PCR and the reporter TOP-Luc were used to determine the expression of β-catenin and the the β-catenin/TCF4 activity of TE85 cells.The cell migration and invasion were detected by scratch wound assay, and Transwell, respectively. DAPI staining and FCM were performed to detect cell apoptosis. Results The endogenous expression of β-catenin of TE85 cells was relatively lower in osteosarcoma cell line compared with other osteosarcoma cells. The mRNA expression level of β-catenin was increased after infection, as well as the activity of β-catenin/TCF4. Scratch test and Transwell results showed that the migration and invasion capacity of cells significantly decreased after Adβ-catenin infection (P<0.05). However, the overexpression of β-catenin resulted in no significant changes in cell apoptosis. Conclusion β-catenin can effectively inhibit the migration, invasion ability of the TE85 cells, but does not affect on its apoptosis. Our findings suggest that β-catenin may be providing a new target for the treatment of osteosarcoma.

Key words: Key words:β-catenin, TE85, Migration, Invasion, Apoptosis

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