Abstract: Objective To investigate that Ano2 is the molecular identity of calcium-activated chloride channels. Methods The full length coding sequence of Ano2 was amplified by RT-PCR，Ano2 and eukaryotic expression vector pEGFP-N3 were ligated；Then recombinant plasmids were transfected into FRT cells using liposome，and the stable transfection FRT cells were selected with antibiotic; the expression and location of Ano2 was observed by the inverted fluorescence microscope and its expression was detected by western blot；the electrophysiological properties of Ano2 were researched by whole-cell patch-clamp technique. Results pEGFP-Ano2 was constructed successfully，FRT cell membrane expressed Ano2；The electrophysiological properties of Ano2 was Ca2+-、time- and voltage-dependent，and an outward-rectifying I/V relationship was observed. Conclusion Ano2 is the molecular identity of calcium-activated chloride channels.

Key words: Key words：Ano2, FRT cells, transfection, calcium-activated chloride channels