Abstract: Objective To explore the expressions of Wnt2 in human hepatocellular carcinoma(HCC) and the suppressive effect of knocking down Wnt2 expression by RNAi on Hepatocellular cell line HepG2. Methods Real-time quantitative PCR (RT-qPCR) method and immunohistochemistry methods was used to detect the expression of Wnt2 in HCC, paired Paracancerous tissue and normal liver tissue;After RNAi was used to knock down the expression of Wnt2 in HepG2,testing the change of Wnt2 and cell proliferation level and cycle change. Result 1).Wnt2 expressed in the highest in cancer, paired Paracancerous tissue is the second smallest,and normal liver minimum. 2). Using RNAi technology,The expression of Wnt2 was downregulated.HepG2 proliferation was inhibition and the cell growth mainly detained in G0/G1 and the percentage of S decreased. Conclusion Wnt2 was over expression in Hepatocellular Carcinoma;siRNA can inhibit expression of Wnt2 as well as the growth of HepG2.Wnt2 may be a novel target in gene regulation of Hepatocellular Carcinoma.

Key words: hepatocellular carcinoma, Wnt2, RNA interference