Abstract: Objective To explore the potential mechanism of DJ-1 via antagonizing ROS-induced Beclin-1 up-regulation on HL-1 cardiomyocytes during hypoxia-reoxygenation. Methods DJ-1 down-regulation was induced in mouse HL-1 cardiomyocytes by lentivirus transfection. The expression of DJ-1，LC3 and Beclin-1 protein expression change after hypoxia-reoxygenation and lentivirus transfection were detected by Western blotting, autophagosomes were detected by MDC, ROS levels were detected by DCFH-DA, Cardiomyocytes death was detected by AnnexinⅤ and PI. Results Compared to the control group cells，significant down-regulation of protein levels of DJ-1 was observed in cardiomyocytes with shDJ-1 virus，while protein levels of Beclin-1 and LC3-Ⅱ in cardiomyocytes with shDJ-1 virus were up-regulated after hypoxia/reoxygenation, NAC decreased protein levels of Beclin-1 and LC3-Ⅱ in cardiomyocytes with shDJ-1 virus. ROS and autophagosome levels were significantly increased after hypoxia/reoxygenation, and apoptoses of those were significantly increased after hypoxia/reoxygenation in cardiomyocytes with shDJ-1 virus, and NAC could reverse of these. Conclusion The effect of DJ-1 via antaganizing ROS induced Beclin-1 up-regulation might involve in the ischemia-reperfusion injury.

Key words: Ischemia-Reperfusion, Autophagy, Apoptosis, DJ-1, ROS