Abstract: Objictive To determine the role of the over-expression of SIL-6R and GP130 on oncological differention of bone marrow mesenchymal stem cell（MSCs）by indirectly co-culturing MSCs and C6 glioma cells. Methods The combination of transwell insert culture dish and 6-well plates was used to build indirectly co-cultured system to simulate the microenvIronment of MSCs growth. GP130 in each group was measured by RT-QPCR and immunofluorescence, STAT-3, CyclinD1 and BCL-xl in each group was measured by RT-QPCR and Western blot, SIL-6R in each group was measured by ELISA, the cell morphological changes were observed by the phase contrast microscope. Results Experimental group cells showed tumor cell-like morphology. Compared with negative control group and blank group, GP130, STAT-3, CyclinD1 and BCL-xl mRNA and protein in experimental group were significantly increased (P<0.05), and there were no significant differences between experimental group and positive control group. The expression rate of GP130 reached 93%±5% in experimental group, while in both negative control and blank group was 0%(P

Key words: bone marrow mesenchymal stem cells, SIL-6R, GP130