Abstract: Engineered nucleases have been successfully applied to a variety of cells and organisms. The programmable engineered nucleases, with sequence-specific cleavage activity, enable a broad range of genome editing by inducing DNA double-strand breaks that stimulate DNA repair at specific genomic locations.

Key words: Engineered nucleases, Genome site-specific editing, Zinc-finger nuclease (ZFN), Transcription activator-like effector nuclease (TALEN), CRISPR/Cas, Engeered meganuclease