Basic & Clinical Medicine ›› 2013, Vol. 33 ›› Issue (10): 1297-1301.

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Lipopolysaccharide Pre-treatment Increases Expression of HIF-1α in Cobalt chloride-induced murine macrophages

  

  • Received:2012-12-26 Revised:2013-03-26 Online:2013-10-05 Published:2013-09-25

Abstract: Objective To observe the effects of hypoxia simulated by cobalt chloride on the expression of hypoxia-inducible factor-1α(HIF-1α) and vascular endothelial growth factor(VEGF) in murine macrophage Raw264.7 induced beforehand by using LPS . Methods Raw264.7 were divided into control group,LPS(1mg/L)group,CoCl2(100μmol/L)group and LPS pre-treatment(LPS-Pre) group and incubated respectively in the culture containing corresponding reagent. The macrophages of LPS pre-treatment group were at first incubated for 8 hours in the culture with LPS of 1mg/L and then were transferred into the culture containing CoCl2 of 100μmol/L. HIF-1α and VEGF’ mRNA were detected by RT-PCR;protein of HIF-1α and VEGF were analyzed by Western blot and immunocytochemistry. Results Compared with those of control, mRNA and protein expression of both HIF-1α and VEGF can be up-regulated in CoCl2 group and LPS-Pre group(p<0.05).Moreover, cells were pre-treated with LPS and followed activated by CoCl2, HIF-1α’s protein significantly higher(p<0.05)than that of induced only by CoCl2. Conclusion Pre-treatment with LPS can up-regulate HIF-1α and VEGF’s protein at the post-transcriptional level in macrophages under hypoxia environment.

Key words: Key words: hypoxia, inflammation, LPS, CoCl2, HIF-1α

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