Abstract: ABSTRACT Objective To search the protein as to human hepatocellular carcinoma MDR and provide evidence for the mechanism study of hepatocellular carcinoma MDR.Methods Human hepatocellular carcinoma cell line Bel-7402 and 5-FU resistant cell line Bel-FU were cultured in vitro.The MDR of Bel-FU was detected by MTT assay,the screen the differential expression protein between Bel-7402 and Bel-FU by 2-DE and MALDI-TOF/ TOF, then Western-blot checked the result . Results compared with those in Bel-7402, 24 proteins were up-regulated in Bel-FU including FAK、TM3、ORP150、CRT、NSE 、80K-H protein、EF-1-D、phosphoprotein and so on ,and 12 proteins were down-regulated. Western blot confirmed that FAK、CRT were up-regulated in Bel-FU, they were in accordance with the results of 2- DE.Conclusion The differential expression protein between Bel-7402 and Bel-FU pay a way for the study of molecule mechanism as to MDR.

Key words: Key words: hepatocellular carcinoma, Multidrug Resistance, Comparative proteomics, 2-DE