Basic & Clinical Medicine ›› 2012, Vol. 32 ›› Issue (5): 500-504.
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Abstract: Objective To evaluate the expression level of microRNA-125b (miR-125b) in gastric cancers and study its roles in cell proliferation and apoptosis. Methods The expression of miR-125b was detectable by real-time PCR in 40 gastric cancer tissues and paired cancer-adjacent tissues. The synthesized miR-125b mimic was transfected into cultured gastric cancer cell lines HGC-27 and MGC-803. The cell proliferation and apoptosis were measured by CCK-8 and flow cytometry respectively. Results MiR-125b was significantly up-regulated in gastric cancer tissues compared with cancer-adjacent controls(P<0.01). Ectopic expression of miR-125b in HGC-27 and MGC-803 cells increased cell proliferation, whereas decreased cell apoptosis. Cells transfected for 72h, HGC-27(scramble:1.632±0.09,mimic:2.473±0.08),MGC-803(scramble:1.603±0.05,mimic:2.554±0.07)),Conclusion MiR-125b may act as an oncogene in gastric cancer via interfering with cell proliferation and apoptosis.
Key words: Keywords: microRNA-125b, gastric cancer, cell proliferation, cell apoptosis
CLC Number:
R735.2
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URL: http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/
http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2012/V32/I5/500