Abstract: Objective To evaluate the effect of microRNA-373（miR-373） on osteogenic differeniation of human adipose tissue-derived Flk1+ mesenchymal stem cells (MSCs). Methods Flk1+MSCs were transfected with miR-373 mimics using Lipofectamin2000 and then induced to differentiate into osteoblasts. The osteogenic efficiency was detected by ALP staining and Alizarin red staining as well as maker genes expression through real-time PCR. Results The ALP staining and Alizarin red staining showed significant difference between two groups. Compared to control group (NC group), the expression of osteogenic maker genes RUNX2 (0.543±0.021), ALP (0.556±0.024) and OC (0.499±0.017) were significantly reduced in the mimics-transfected group (miR-373 group) (P<0.05). Conclusion miR-373 could regulate the osteogenisis of Flk1+ MSCs negatively.

Key words: miR-373, MSCs, osteogenisis