Abstract: Object To investigate the effect of miR-424 on adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hAMSCs). Methods Expression level of miR-424 during adipogenic differentiation of hAMSCs was detected by Real-Time PCR. hAMSCs were transfected with synthetic miR-424 mimics using siPORT NeoFX Transfection Agent to increase the level of miR-424 in hAMSCs, and then cultured in adipogenic differentiation medium. Oil red O staining was used to observe the accumulation of lipid droplets in hAMSCs. Expression levels of key adipogenic transcription factor PPARγ and adipogenic-related genes were detected by Real-Time PCR and Western blot. Results The expression of miR-424 decreased after hAMSCs were induced to differentiate into adipocytes. Compared to control group, the level of miR-424 was significantly increased in miR-424-mimics-transfection group. Besides，decreasing of lipid droplets accumulation was observed by Oil red O staining on Day 8 of adipogenic differentiation. Moreover, the levels of PPARγ and adipogenic-related markers were also down-regulated in the miR-424-mimics transfection group. Conclusion miR-424 could negatively regulate adipogenic differentiation of hAMSCs.

Key words: miR-424, hAMSCs, adipogenic differentiation