Abstract: Objective To investigate the protective effects and mechanisms of peroxisome proliferators activated receptor (PPAR)-α agonist Wy14643 on HL7702 cells injured by H2O2. Methods The mRNA and protein expressions of PPAR-α in H2O2-damaged HL7702 cells were examined by RT-PCR and Western blot respectively. Pretreated with Wy14643 before H2O2 exposure, cell viabilities, superoxide dismutase (SOD) and catalase (CAT) activities, and NF-κB activation in HL7702 cells were detected. Results PPAR-α mRNA and protein expressions decreased by H2O2 exposure. After 200 and 300 μmol/L H2O2 exposure, the protein expression levels of PPAR-α reduced from 0.69±0.04 to 0.48±0.05 and 0.38±0.03 respectively (P<0.01). Pre-incubated by Wy14643, the decreased cell viabilities, SOD and CAT activities, and increased NF-κB activation in H2O2-damaged HL7702 cells were all inhibited. Conclusion The present study provides evidence that Wy14643 alleviates oxidative damage in HL7702 cells induced by H2O2 and enhances the cellular antioxidant capacity, which may be associated with the inhibition of NF-κB activation.

Key words: PPAR-α, Wy14643, H2O2, HL7702 cell, oxidative stress