Abstract: Objective Observe the effect of silencing JAB1 gene expression on human hepatoma cells proliferation. Methods By means of the RNA interference (RNAi) technique, the pAVU6-JAB1 siRNA interference vectors were constructed and transfected into hepatoma cell line HepG-2. The Jab1 expression was measured using real-time PCR and weatern-blot methods. The cells proliferation was measured using WST-8 methods. Results 96 hours after transfection of the PAVU6-JAB1 siRNA vectors, the proliferation of hepatoma cell line were inhibited from 99.6?1.4%(empty vector transfection group) to 70.8?1.6%（experimental group）. The Jab1 expression was also inhibited，whereas the P27kip1 expression level was significantly elevated. Conclusion Inhibition of the expression of the JAB1 protein in hepatoma cells could inhibit hepatoma cell proliferation to some extent.

Key words: JAB1, RNA interference, hepatoma cell, proliferation