Abstract: [Abstract] Purpose: We use recombinant Ad-ING4 and Ad-PTEN to infect human hepatocarcinoma cell line SMMC-7721 in vitro, test and compare the effects of gene expression of the cells. Method: We use recombinant Ad-ING4 and Ad-PTEN and control virus to infect QBI-293A cells for viral propagation and viral titer detection. We confirm the infective effects of Ad-ING4, Ad-PTEN and control virus by fluorescence observation. Use Ad-ING4 and Ad-PTEN to infect SMMC-7721 cells and confirm their effects by their apoptotic rate and the results of MTT. Results: The apoptotic rate of control virus group is 3.4±1.3%, the Ad-ING4 group is 49.7±4.8%（p＜0.01）and the Ad-PTEN group is 11.4±3.2%（p＜0.01）. The cell proliferation of group Ad-ING4 and Ad-PTEN obviously decreased from 48h to 96h（p＜0.05）.Conclusion: The data analysis showed Ad-ING4 and Ad-PTEN had an inhibitory effect to SMMC-7721 cells, and the effect of Ad-ING4 is more severe.

Key words: hepatocarcinoma, Ad-ING4 , Ad-PTEN, gene