Basic & Clinical Medicine ›› 2011, Vol. 31 ›› Issue (12): 1326-1329.
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Abstract: Abstract: Objective:To investigate the effects of the recombinant angiotensin-converting enzyme 2(rACE2)gene on Profilin-1 expression and cell proliferation in vascular smooth muscle cell. Methods: The cultured human umbilical arterial smooth muscle cells (HUASMC) were stimulated by Ang II in the presence and absence of rACE2 gene, the HUASMC proliferation and Profilin-1 expression level were determined with MTT, real-time PCR and Western blotting,respectively. Results: Compared with the control, Profilin-1 expression were significantly increased in HUASMC (3.50±0.30 vs. 1.00±0.10; n=5~6, P<0.05) by stimulating with Ang II(100 nmol /L)for 6 hours, which were inhibited by rACE2 gene intervention (1.73±0.12 vs. 3.50±0.30; n=5~6, P<0.05). Conclusion: ACE2 gene over-expression contributes to the reversion of the Ang II- induced Profilin-1 expression and proliferation in human umbilical arterial smooth muscle cells.
Key words: Key words: angiotensin-converting enzyme 2, Profilin-1, hypertension, angiotensin II, cell proliferation
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中图分类号:R.544.109
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http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2011/V31/I12/1326