Abstract: Objective To investigate the effects of calorie restriction on vascular senescence and underlying mechanisms. Methods Comparing ageing-related gene expression in aortas of 2 or 18-month-old C57BL/6 mice by western blotting. Constructing calorie restriction mouse model and analyzing gene expression by western blotting using ageing-related antibodies. Results Ageing decreased the expression of SIRT1, a type III histone deacetylase, in aortas of mice. Whereas long-term calorie restriction increased SIRT1.The ageing-related biomakers P53 and P21 expression were decreased, but MnSOD expression was upregulated in aortas of C57BL/6 mice by long-term calorie restriction. Conclusion Long-term calorie restriction inhibits vascular senescence. SIRT1 is involved in vascular senescence inhibition, leading to decreased oxidative stress.