Abstract: Objective To discover biological markers. Methods Two-dimensional electrophoresis and Mass-Spectrum techniques (MS+MS/MS) were used for analyzing the seminal plasma from idiopathic male infertility and control. Results Serum albumin and prostatic acid phosphatase were reduced in seminal plasma from idiopathic male infertility rather than the control, while Cathepsin B and Zn-alpha-2-glycoprotein were increased. Conclusion Idiopathic male infertility possibly was associated with disorder of sperm capacitation and seminal immune function.

Key words: Idiopathic male infertility, Seminal plasma, Proteomics