Abstract: Objective The present study aimed to investigate the effect of PP60C-SRC on the viability of rat spermatogonial stem cells in 9 day-old rat. Methods MTT method was used to observe the viability of the spermatogonial stem cells treated with antisense C-SRC oligodeoxynucleotides (ODNs) and PD98059 in vitro; Western blot was used to observe the PP60C-SRC and phosphorylated extracellular regulated kinase1/2 (p-ERK1/2) contents. Results Compared with that in control group, the viability of the cells decreased by 8.1 % (P<0.05) and 9.4 %(P＜0.01), respectively, after the spermatogonial stem cells were treated with antisense C-SRC ODNs and PD98059. Compared with the control group, the PP60C-SRC and p-ERK1/2 contents decreased by 33.8 % and 38.4 % (both P<0.01), respectively, in the spermatogonial stem cells after transfected antisense C-SRC ODNs. Compared with the control group, the p-ERK1/2 contents decreased by 34.5 % (P<0.01) in the spermatogonial stem cells after treated with PD98059. Conclusion PP60C-SRC regulates the viability of rat spermatogonia stem cells through p-ERK1/2.

Key words: rat, spermatogonial stem cells, pp60c-src, ERK1/2