Abstract: Object To clarify the relationship between CT120, a novel human plasma membrane-associated gene, and prolifetation of lung adenocarcinoma cell line A549. Method Expression vector（pcDNA3.1）containing antisense oligonucleotides of CT120 was constructed and transfected into the lung adenocarcinoma cell line A549. RT-PCR and Western blot detected the expression of CT120. Meantime, flow cytometry and soft agarose colony formation analyzed the cell proliferation, and P53、CYCLIND1 and CDK4 were detected by RT-PCR. Result pcDNA3.1 containing antisense oligonucleotides of CT120 was constructed successfully and could inhibit the expression of CT120 effectively by RT-PCR and Western blot. The expression of P53 was up-regulated and the expression of CYCLIND1、CDK4 were down-regulated. Conclusion The down-regulation of CT120 expression by antisense oligonucleotides technique may be a new candidate of drug target for treatment of lung cancer.

Key words: CT120, Antisense oligonecleotides, lung cancer