The crystallization of neural RNA binding protein Musashi1 RRM1

Basic & Clinical Medicine ›› 2009, Vol. 29 ›› Issue (4): 337-341.

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The crystallization of neural RNA binding protein Musashi1 RRM1

Xian-ping WANG, Li ZHAO, Jing-xi ZHU,

National Lab of Medical Molecular Biology, Institute of Basic Medical Science& School of Basic Medicine, CAMS & PUMC National Lab of Medical Molecular Biology, Institute of Basic Medical Science& School of Basic Medicine, CAMS & PUMC IBMS，CAMS，School of Basic Medicine PUMC National Lab of Medical Molecular Biology, Institute of Basic Medical Science& School of Basic Medicine, CAMS & PUMC National Laboratory of Medicine Molecular Biology, IBMS, CAMS，School of Basic Medicine PUMC

Received:2009-01-06 Revised:2009-01-15 Online:2009-04-25 Published:2009-04-25

Abstract

Abstract: Objection To clarify the structure of important function domain of Musashi1-RRM1, crystallized the Musashi1 RRM1 domain. Mothods The Musashi1 RRM1 was chosen to be expressed, in the E.coli BL21(DE3), purified by ion exchange and gel filtration, and crystal screen used the Hampton Research Kit and optimize the crystallization condition. Results The initial crystal of Musashi1 RRM1 was obtained at last. Conclusion The crystallization of Musashi1 RRM1 is satisfied the following data collection demand.

Key words: RNA binding protein, Musashi1RRM1, protein expression and purification, crystallization

CLC Number:

R392.1

Xian-ping WANG; Li ZHAO; Jing-xi ZHU; . The crystallization of neural RNA binding protein Musashi1 RRM1[J]. Basic & Clinical Medicine, 2009, 29(4): 337-341.

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The crystallization of neural RNA binding protein Musashi1 RRM1

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