Abstract: Object To investigate the protective role of FDP in STZ induced islest apoptosis and to explore the mechanisms of the protective effect of FDP. Methods:The pancreases of the rats were removed to collect islets cells.The cells were incubated with STZ with/or FDP. Cell ,insulin secretion ,HO-1 activity ,CO content ,SOD activity ,GSH-px activity, iNOS activity, No conetent and apoptotic percentage were detected. Results: HO-1 activity and CO content of the normal control group were low. STZ induced a significant decrease of cell activity and insulin release, flow cytometry analysis showed that apoptotic percentage of islet cells remarkably increased following the addition of STZ, FDP obviously improved the the islets cellular activity damaged by STZ,and basic amount of insulin secretion and stimulated by high glucose were improved（P＜0.01）. Content of CO , activity of HO-1, SOD activity ,GSH-PX activity were lower in the STZ group than the normal control group（P＜0.01）,and there were significant differences between conetent were higher in the STZ group than in the FDP group.FDP decreased cell the FDP groups and STZ group （P＜0.01 or P＜0.05）. iNOS activity and No apoptotic percentage（P＜0.01 or P＜0.05）. Activities of HO-1 and content of CO were higher than in the STZ group （P＜0.01 or P＜0.05）. Conclusion: FDP can attenuate the STZ induced apoptosis of culturedβcells.the mechanisms may be improved HO-1 activity and CO content, also improved SOD activity ,GSH-px activity and decreased iNOS activity and NO content.

Key words: FDP, STZ, apotosis, islets cells, Heme oxygenase-1, carbon monoxide, Inos, NO, SOD, GSH-PX