Abstract: Objective To speculate on the function of the human D-Tyr-tRNATyr deacylase (h-DTD)It's influence on nonselective cation channels permeability of Na+, K+ and Ca2+ in neuroblastoma SH-SY5Y cell line. Methods The enzyme activity of h-DTD was analyzed with capillary electrophoresis; then the h-DTD protein expression level was detected in different tissues and cell lines with western blot. Also, the membrane patch clamp technology was used to test the whole-cell potential change in SH-SY5Y cell line. Results h-DTD has deacylase activity in vitro, and this protein is expressed widely in different tissues. however, it is enriched in brain. As we have found that the L-glutamic acid sensitive channel could be motivated by D-amino acid and L-glutamid acid only in the presence of h-DTD and resulted in the iron permeability enhanced. Conclusion h-DTD could affect the nonselective cation channels through the regulation of dissociative D-form amino acid concentration, which implies it may function as a regulator.

Key words: h-DTD, expression pattern, the nonselective cation channels