Abstract: Objective To screen the proteins interact with the cytoplasmic tail of NECL1. Methods The cytoplasmic tail sequence of human NECL1 gene was inserted into the "bait" vector pGBKT7 in frame. The recombinant pGBKT7-NECL1C was then transformed in to yeast cells and followed by screening of human fetal brain cDNA library. Then the GST pull down assay was used to confirm the interactions between the proteins. Results Nine different sequences by sequencing the positive clones(There are repeated clones) and five alternative proteins were obtained by the amino acid sequence blast. Using the GST pull down analysis, we confirmed that two of them can interact with the cytoplasmic tail of NECL1 in vitro. Conclusion Using yeast two-hybrid system, we got some alternative proteins which may interact with the cytoplasmic tail of NECL1.

Key words: yeast two-hybrid, NECL1, interaction protein