Abstract: Objective Establishment and characterization of healthy donor's γδT cell clones. Methods γδT cells were cloned by limiting dilution after positive sorting, with 60Co irradiated allogeneic PBMC as feeder cells. Flow cytometry analysis and molecular biology technique were then used to identify γδT cell clones, and MTT assay was used to verify their proliferation after incubated with epitope peptides recognized by γδT cells. Results A γδT cell clone had been established. The subtype of this clone was V γ9 V δ2 and the expression of CD4 and CD8 molecule were negative. Further studies indicated that epitope peptide EP6 could not only specifically bind γδT cell clone but also trigger the proliferation of γδT cell clone. Conclusion A healthy donor's γδT cell clone was successfully established, which laid a solid foundation for further study on γδT cells.

Key words: γδT cells, clone, limit dilution