Abstract: Objective To observe the effect of tyrosine kinase Src on activating Notch-1 protein in pancreatic cancer cell line - BxPC3. Methods Notch-1 expression and c-Src expression were inhibited by siRNA interference, and the activation of Src was inhibited by its inhibitor PP2. The growth rate of BxPC3 cells was measured by MTT. The changes of Notch-1 NICD level and Src protein level were measured by Western blot. Results The growth of BxPC3 cells decreased after inhibiting Notch-1 expression, as well as the cells were treated with PP2. The level of Notch-1 NICD decreased both after inhibiting c-Src expression and after Src activation was inhibited by PP2. Conclusion In BxPC3 cells, tyrosine kinase Src promotes the level of Notch-1 NICD, which directly influences the cell growth.

Key words: pancreas cancer, BxPC3, Notch-1, Src, PP2