Abstract: Objective To evaluate the nourishment and immune privilege effects of Sertoli cells to co-encapsulated hepatocytes. Methods The hepatocytes and Sertoli cells were alone encapsulated or co-encapsulated in various ratio of 100:1、50:1、20:1、10:1, and co-cultured for 21 days in vitro. The secretion of albumin and urea was analyzed, and the morphology of encapsulated cells was observed by microscopy, then to determine the best mixed ratio of hepatocytes to Sertoli cells. Splenocyte proliferation response was assessed to evaluate Sertoli cell's immune privilege function to hepatocytes by CCK-8. Results Sertoli cells could elevate hepatocyte's secretion of albumin and urea when they were co-encapsulated with each in appropriate ratio (P<0.01), and prolonged the survival time of hepatocytes. The mixed ratio of 20:1 get the best effect at secretion of albumin and urea . Capsules contained hepatocytes initiated significant splenocyte proliferation response, in spite of more significant to hepatocytes group (P<0.01). Sertoli cells could inhibit splenocyte proliferation obviously. Conclusion Co-encapsulated hepatocytes with Sertoli cells is an available approach to sustain hepatocyte's function、morphology and survival, and inhibit impairment of immune response partially.