Abstract: RNA interference (RNAi) is a process that functions to inhibit the specific gene expression via the degradation of the target mRNA induced by the double strand RNA. The use of RNAi in mammals as a tool to study gene function has rapidly developed in recent years. Here we described the novel progress and applications of the vector for mediating RNAi in mammals.

Key words: RNA interference, small interference RNA, nonviral vector, plasmid vector