Abstract: Objective 　To study the effect of -1 site single nucleotide polymorphism ( SNP) on CCNH gene promoter transcription activity. Methods　PCR and site-directed mutagenesis technology were used to construct CCNH basic promoter and -1G mutate promoter. Dual-Luciferase Reporter assay system was used to detect the transcription activity of constructed promoter. Results 　In AD293 cells, the activity of - 1G mutate type promoter was significantly lower than wild type -1T promoter（P<0.05）. Conclusion　It is implied that SNP-1T→G base variation can decrease the transcription activity of CCNH gene promoter.

Key words: CCNH gene, SNP, Dual-Luciferase Reporter assay system