Abstract: Objective　To investigate the effect of Notch1 and Rab5 on the growth of pancreatic cancer BxPC3 cells and the regulation of Notch1 activity by Rab5 in BxPC3 cells. To investigate the effect of Wortmannin and LY294002 on the growth of pancreatic cancer BxPC3 cells and the regulation of Notch1 activity. Methods　Notch1 and Rab5 expression were inhibited by siRNA interference. The growth rate of BxPC3 cells was measured by MTT. Rab5 expression was inhibited by siRNA interference ,the changes of Rab5 and Notch1 protein were measured by Western blot . Results　The growth rate of BxPC3 cells decreased after inhibiting Notch1 and Rab5 expression. Notch1 protein level decreased after inhibiting Rab5 expression. After adding Wortmannin and LY294002, the growth of BxPC3 cells and Notch1 protein level decreased . Conclusion 　In BxPC3 cells, Notch1 and Rab5 are important to cell growth. Rab5 may regulate Notch1 activity. Wortmannin and LY294002 are important to cell growth and regulate Notch1 activity.

Key words: pancreatic cancer, BxPC3, Notch1, Rab5, Wortmannin, LY294002