Abstract: Abstract: Objective: We have showed the stronger ability to migrate through human brain microvascular endothelial cells (HBMECs) in peripheral T lymphocytes of AD patients than age-matched is associated with a significantly higher macrophage inflammatory protein-1α (MIP-1α) expression of T lymphocyte. In order to explore the mechanism of the transendothelial migration of T lymphocytes further, we choose 6T-CEM cells expressing MIP-1α as a model cell line of AD patients’ T lymphocytes to investigate this mechanism. Methods :Immunofluorescence, western blot, plasmid construction and transfection, transendothelial migration and endothelial permeability assay are used to explore the progress of the transendothelial migration of T cells. Results: The expressions of CCR5 on HBMECs which incubated with 6T-CEM cells were detected. The induced expressions of CCR5 can enhance the migration of 6T-CEM cells through HBMEC monolayer. Conclusion: CCR5 on HBMECs might be involved in the transendothelial migration of T lymphocytes .

Key words: CCR5, 6T-CEM cells, HBMEC