Abstract: Objective To explore a method for establishing a stable auditory deprived animal model of mouse. Methods Forty two-month old BALB/c mice with normal auditory brainstem response (ABR) thresholds were divided into two groups randomly. The experimental group mice were underwent an incision inferior to the pinna, and the cochleas were destoried with a drill through the bulla bilaterally. The control group mice were underwent only an incision without cochlea ablation. Mice were tested for ABR thresholds 4 month later to assess hearing sensitivity by comparing the ABR before and after operation. Results In the experimental group, mice's auricle reflection disappeared after operation and ABR waves couldn't be detect. In the control group, the tests of mice's hearing were normal. Conclusions Mouse cochlea ablation is a reliable method for establishing a stable auditory deprived animal model of mouse by the pinna inferior approach. It can be used to research auditory centre plasticity induced by auditory deprived.