Abstract: Objective To compare the inducing effect of three kinds of connective tissue from amnion, fetal mesentery and intestinal submucosa on the differentiation of fetal BMSCs in vitro. Methods The epithelium on three kinds of connective tissue were removed. and EGF and IGF-1 in these tissue clumpes were detected .The DAPI-P3-BMSCs were planted on the surface of three kinds of connective tissue to culture. There were connective tissue induced groups, connective tissue and EGF and IGF-1 induced groups, EGF and IGF-1 induced as control group. The tissue clump was collected on cultured 8d、10d、12d, to compare their inducing effect on the differentiation of BMSCs. Results All of BMSCs induced by three kinds of connective tissue and growth factors could express CK or EMA. The effect of unite-induced groups was stronger than those groups induced only by connective tissue, P＜0.05; The strongest inductive effect of three connective tissue was exerted by amnion, P＜0.05. EGF was expressed in the three kinds of connective tissue, and IGF-1 was only expressed in mesentery and intestinal submucosa. Conclusion BMSCs could be induced to differentiate into epithelial cells by the connective tissue of amnion, mesentery and intestinal submucosa, EGF and IGF-1 in vitro; EGF, IGF-1 could increase the effects of these connective tissue on the induction of BMSCs differentiation; EGF and IGF-1 may be one of the endogenic factor in these connective tissue which induces BMSCs to differentiate into epithelium.

Key words: Fetus, Connective tissue, Bone marrow-derived mesenchymal stem cells, Induction, Differentiation