Abstract: Objectives To find the way of inducing the bone marrow mesynchymal stem cells（MSCs）into cardiac cells with pacemaking function in vitro. Methods Dissociate the rat MSCs and induce them with 5AZA, bFGF+EGF, HGF, SCF, lysate of the sinoatrial cells respectively. The morphological changes were observed，and the expressing of protein cTnT，connexin 43 and HCN2/4 were analyse by immunohistologic and flowcytometry techniques.The pacmaking current If were evaluted by patch clamp techniques. Results All the methods can induce the bone marrow MSCs to differentiate into cardiac cells, which expressing cardiac cell specific protein and HCN2. The results shows that cells induced by 5AZA, bFGF+EGF and SAN CMs show higher rate of HCN2 expressing (22.9%,22.3%,11%). The cells of these groups have the pacmaking current If. Conclusions Lysate of the sinoatrial cells are the ideal methods of inducing the bone marrow MSCs to differentiate into cardiac cells with pacemaking function in vitro.HCN is a promising marker protein to select pacmaking cells out of the differentiated cells.