Abstract: Objective To dynamically observe changes of cytokines of splnocytes in mice by immunization with recombinant BCG-EmII/3 vaccine of Echinococcus multilocularis(Em).Methods BALB/C mice were intranasally vaccinated by the vaccine and killed to get spleen on 0、2、4、6、8 and 10w of immunization respectively, splenocytes were separated to culture by stimulation with EmAg or ConA，their supernatants were gathered to measure level of IL-2,IFN-γ,TNF-α and IL-4 by ELISA Kits. PBS was served as control. Results In the groups of immunization, levels of IL-2,IFN-γ,TNF-α and IL-4 increased obviously on 2～6、2～6、2～10 and 8～10w respectively, reached the highest level on 4、4、8 and 10w respectively. Conclusion TH1 response was induced in mice by rBCG-EmII/3 vaccine on early immunization（2～8w）.