Abstract: Objectives To determine the diagnostic value of antifilaggrin antibodies(AFA) in rheumatoid arthritis(RA) and compare the correlation among AFA，anti-perinuclear factor(APF) and anti-keratin antibodies(AKA). Methods Filaggrin was extracted from human epidermis and partially purified. AFA in 103 cases of RA and 140 cases of controls were detected by western blot. APF and AKA were detected using indirect immunofluorescence. Results AFA test resulted in diagnosis of 35.9% of 103 RA samples, with a specificity of 93.7%. The result was significantly higher than the controls (P<0.001). 26 (70.3%) samples out of 37 AFA positive sera were positive in the AKA test and 31 samples out of 37 AFA positive were positive in APF test. The data show that there is a positive correlation among AFA, APF and AKA tests. Conclusion AFA has diagnostic value in rheumatoid arthritis. Although there is a positive correlation among AFA, APF and AKA, AFA dose not replace APF and AKA tests.