Abstract: Objectives To develop an analyzing technique of single nucleotide polymorphism(SNP) based on non-probe real-time quantitative PCR, and to study the relationship between SNP in human adiponectin (APM1) gene and type 2 diabetes (T2DM). Methods Design primers with specific 3'-terminal nucleotides and perform Real-time PCR using SYBR Green dye. Genotypes were determined by comparing the amplification efficiency of each pair of primer and verified by sequencing. SNPs 45 and 276 in AMP of 20 T2DM, 24 obesity and 28 healthy persons were analyzed with this technique. Results This technique could discriminate genotype as efficient as sequencing. The genotype of APM1 at +45 site was significantly correlated with T2DM (p<0.05). Moreover, the relative risk of T2DM incidence in persons with TT genotype was 3 times of those with GG and GT. On the contrary, obesity was not correlated with SNP at +45 nor +276 sites of APM1 gene. Conclusion Allelic 3'-terminal specific primers real-time PCR could be used to determine SNPs. SNP at +45 sites of APM1 gene correlated with T2DM.