Abstract: Objective To identify certain isoforms involved in the onset of retinal ischemic preconditioning (IPC), the effects of ischemic pretreatment (IP) were observed on levels of conventional protein kinase C (cPKC) a, bI, bII and g isoform-specific membrane translocation and protein expression in retina of rats. Methods Retinal IP was produced by intra-ocular pressure (IOP) elevation for 5 minutes in anesthetized Wistar rats. Sham operation was similar to IP except the pressure elevation. 10, 20, or 40 minutes and 1, 12, 24, 72, or 168 hours after the procedure, levels of cPKC isoform-specific membrane translocation and protein expression were analyzed using Western-blot. Results cPKCa protein expression levels increased significantly from 12h to 168h. A peak reached at 72h after IP. cPKCg membrane translocation enhanced during 20min to 1h with a peak at 40min. cPKCg protein expression levels increased significantly from 12h to 72h. A peak was at 24h after IP. However, there were no significant changes in both membrane translocation of cPKCa, bI, bII and protein expression of cPKCbI, bII in retina of rats following IP. Conclusion The enhanced cPKCg membrane translocation, and the increased cPKCa and g protein expressions might be involved in the onset and sustain of retinal IPC in rats respectively.