Abstract: Obiective:To study whether focal adhesion kinase (FAK) prompte human pulmonary artery smooth cells (HPASMCs) proliferation .Method: Cultured HPASMCs stimulated by fibronectin (40mg/L) were passively transfected with sense -FAK oligonucleotides(ODNs), FAK activity was measured by immunoprecipitation and expression of FAK protein was detected by Western blots .Meanwhile, The change of cell proliferation were measured by MTT and 3H-TdR absorbation experiment. Results: The changes of FAK activity and FAK protein content exhibited dose and time dependence at diferential concentration and time passively transfected with sense-FAK ODNs in Cultured HPASMCs. At the same time, sense-FAK ODNs prompoted HPASMCs proliferation and 3H-TdR absorbation .Conlusion: FAK can faciliate HPASMCs proliferation multiplication,which may play an important function in pulmonary artery hypertension development.