Abstract: Objective To explore the molecule mechanism of protective effects of transfecting human elafin gene recombinant plasmid to NCI-H292 .Methods Through constructing eukaryotic expression vector pEGFP-C1-elafin and transfecting it into NCI-H292 cells ,then co-incubation with polymorphonuclear granulocyte(PMN) and stimulating these cells with lipopolysaccharide(LPS).Afater 24hour,Western bolt and MTT technique were respectively performed to detect expression of ZO-1 in cells and adhesion capability of cells with collagen.Results After stimulation with LPS，Western blot experiment showed that the 220-kD zonula occludens-1(ZO-1) protein in transfecting pEGFP-C1 NCI-H292 cells extracts were obviously decreased，but were still present in transfecting pEGFP-C1-elafin NCI-H292 cells extracts.And MTT technique showed that compared with transfecting pEGFP-C1-elafin cells,adhesion capability of transfecting pEGFP-C1 cells with collagen were obviously decreased.Conclusions Transfection of recombinant human elafin gene into airway epithelial cells could maintain airway epithelium and enhance the capability of airway to inflammatory injury.