Abstract: Objective To evaluate the clinical efficacy of transplantation of autologous peripheral blood stem cells (PBSC) for the treatment of toe or heel ulcer and rest pain in lower limb arterial ischemic diseases. Methods Mobilized patients' own stem cells with G-CSF for 5 days. On the sixth day, PBSC were collected with a blood-cells separator. Later, the PBSC were intramuscularly injected into the ischemic areas of the lower limbs. Results After transplantation, all patients were followed up from 3 to 24 months. The rest pain disappeared in 12 patients，while toe or heel ulcers were cicatrized in 11 cases. However, 4 patients lost. Conclusion Transplantation of autologous peripheral blood stem cells is an effective method for arterial ischemic disease.