Abstract: Abstract Objective: To elucidate the features of the expression of p53, proliferation and apoptosis in gestational trophoblastic tumor (GTT). Methods: 10 cases of normal placenta, 10 cases of complete hydatidiform mole (CM), 20 cases of invasive hydatidiform mole (IM) and 19 cases of choriocarcinoma(CCA) paraffin-embedded tissues were studied. P53 and PCNA were detected with immunohistochemistry and apoptosis was detected with terminal deoxy-nucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL). Results: In NP, CM, IM and CCA, The p53 index(p53-I) in them were 4.12%, 21.68%, 39.61% and 27.39% respectively with significant differences among them (F=6.687, P=0.001). the PCNA index (PI) were 10.40%, 20.76%, 53.60% and 51.95% respectively with significant differences among them (F=17.510, P=0.000). The Apoptosis index (AI) in them were 1.88%, 2.59%, 6.45% and 1.26% respectively with significant differences among them (F=7.020, P=0.000). Significantly positive correlation was found between p53-I and PI（r=0.587，P=0.000）, while there is no correlation between p53-I and AI（r=0.113，P=0.096）. Conclusion: The process of GTT is associated with trophoblastic cell overproliferation because the proliferation and apoptosis are out of control. The increasing expression of muta-p53 contributes to the cell proliferation in GTT and can be helpful to the progress of GTT. No correlation was found between p53-I and AI.