Abstract: Objective To analyze the level of hepatic sterol carrier protein 2 (Scp2) mRNA and bile cholesterol saturation index(CSI) during gallstone information in C57BL/6 mice. Methods Twenty C57BL/6 mice were divided into two groups equally: control group and gallstone group. The gallstone group mice were fed with lithogenic diet (containing 1% cholesterol) for 4 weeks. The mRNA level of Scp2 in liver tissue was measured by RT-PCR. The content of biliary lipids and CSI were analyzed by Automatic Biochemistry Analyzer. Results The levels of Scp2 mRNA and bile CSI were significantly increased in gallstone group. Conclusion A positive correlation between the expression of liver Scp2 and bile CSI was found during gallstone formation.