Abstract: Objective To investigate the feasibility of recombinant adeno-associated virus encoding secrected forms of human acidic fibroblast growth factor transfecting endothelial progenitor cells. Methods sp-haFGF was obtained through combining signal peptide sequence of FGF-4 with native aFGF gene by PCR. Sp-haFGF was cloned into AAV vector plasmid pAAV-IRES-hrGFP . Recombinant AAV encoding sp-haFGF was packaged through co-transfecting HEK293 cells with plasmid sp-haFGF-pAAV-IRES-hrGFP, pAAV-RC and pHelper. Ex vivo cultured EPCs were infected with concentrated rAAV . Expression of green fluorescent protein(GFP) in EPC infected was observed by fluorescence microscope and expression of sp-haFGF in EPCs was verified by RT-PCR and western blot analysis. Results Recombinant