Activin A improves the outcomes of neurological function in mice with ischemic stroke through ACVR1C of oligodendrocytes

doi:10.16352/j.issn.1001-6325.2023.03.380

Abstract

Abstract: Objective The role of activin A receptor type 1C(ACVR1C) in white matter remyelination and neurological outcome of mice with ischemic stroke were investigated. Methods Mice were randomly divided into empty virus vector sham operation group, adeno-associated virus(AAV) sham operation group, empty virus vector model group, AAV model group, empty virus vector activin group A and AAV activin group A.Acvr1c^flox/flox mice with middle cerebral artery oclusion-reperfusion (MCAO/R) for 1 to 10 days were established. Adeno-associated virus AAV-PDGFRα-cre and AAV-PLP-cre with cre recombinase were injected into the lateral ventricles of the mice. Conditional knockout of Acvr1c on oligodendrocytes and oligodendrocytes. Neurological function score was used to observe the prognosis of neurological function. The expressions of ACVR1C protein and myelin basic protein (MBP) were detected by Western blot. Results With the extension of reperfusion time, activin A improved the behavioral outcome of mice by ACVR1C on oligodendrocytes at day 7 (P＜0.05), and significantly increased the expression of MBP protein in corpus callosum at day 10 (P＜0.05). Conclusions Activin A promotes white matter remyelination and improves outcomes of neurological function of ischemic stroke mice by acting on ACVR1C on oligodendrocytes.

Key words: ischemic stroke, activin A, white matter remyelination, activin A receptor type 1C(ACVR1C), outcome

CLC Number:

R743.3

LI Yudie, WANG Hongyu, HAN Song, LI Junfa. Activin A improves the outcomes of neurological function in mice with ischemic stroke through ACVR1C of oligodendrocytes[J]. Basic & Clinical Medicine, 2023, 43(3): 380-385.

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URL: http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/10.16352/j.issn.1001-6325.2023.03.380

http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2023/V43/I3/380

References

[1]Xu S, Lu J, Shao A, et al.Glial Cells: Role of the Immune Response in Ischemic Stroke[J].Front Immunol, 2020, 11: 294.doi: 10.3389/fimmu.2020.00294.
[2]Qin C, Zhou LQ, Ma XT, et al. Dual Functions of Microglia in Ischemic Stroke[J]. Neurosci Bull, 2019, 35: 921-933.
[3]Fern R, Matute C. Glutamate receptors and white matter stroke[J]. Neurosci Lett, 2019, 694: 86-92.
[4]赵翀翀,郭文婷, 蔡宏斌, 等. 大鼠脑缺血-再灌注后G蛋白偶联受体84的表达升高[J]. 基础医学与临床,2021, 41: 398-403.
[5]Morianos I, Papadopoulou G, Semitekolou M, et al. Activin-A in the regulation of immunity in health and disease[J]. J Autoimmun, 2019, 104: 102314. doi: 10.1016/j.jaut.2019.
[6]Zheng J, Zhang T, Han S, et al. Activin A improves the neurological outcome after ischemic stroke in mice by promoting oligodendroglial ACVR1B-mediated white matter remyelination[J]. Exp Neurol, 2021, 337: 113574. doi: 10.1016/j.expneurol.2020.113574.
[7]Dai Q, Li S, Liu T, et al. Interleukin-17A-mediated alleviation of cortical astrocyte ischemic injuries affected the neurological outcome of mice with ischemic stroke[J]. J Cell Biochem, 2019,120:14498-11509.
[8]Roaldsen MB, Lindekleiv H, Mathiesen EB. Intravenous thrombolytic treatment and endovascular thrombectomy for ischaemic wake-up stroke[J]. Cochrane Database Syst Rev, 2021, 12: Cd010995. doi: 10.1002/14651858.
[9]Llorente IL, Xie Y, Mazzitelli JA, et al. Patient-derived glial enriched progenitors repair functional deficits due to white matter stroke and vascular dementia in rodents[J]. Sci Transl Med, 2021, 13: eaaz6747. doi: 10.1126/scitranslmed.
[10]Miron VE, Boyd A, Zhao JW, et al. M2 microglia and macrophages drive oligodendrocyte differentiation during CNS remyelination[J]. Nat Neurosci, 2013, 16: 1211-1218.
[11]Plemel JR, Liu WQ, Yong VW. Remyelination therapies: a new direction and challenge in multiple sclerosis[J]. Nat Rev Drug Discov, 2017, 16: 617-634.
[12]Ibáñez CF. Regulation of metabolic homeostasis by the TGF-β superfamily receptor ALK7[J]. Febs J, 2022, 289: 5776-5797.
[13]Hong X, Wen B, Zhang H, et al. Biological effects of NODAL on endometrial cancer cells and its underlying mechanisms[J]. Exp Ther Med, 2021, 21: 1-10.
[14]Dillenburg A, Ireland G, Holloway RK, et al. Activin receptors regulate the oligodendrocyte lineage in health and disease[J]. Acta Neuropathol, 2018, 135: 887-906.