LncRNA FGD5-AS1 reduces ox-LDL-induced damage of HUVECs by targeting miR-106a-5p

doi:10.16352/j.issn.1001-6325.2022.05.026

Abstract

Abstract: Objective To explore the effect of lncRNA FGD5-AS1 on oxidized low-density lipoprotein (ox-LDL)-induced vascular endothelial cell damage and its regulatory effect on miR-106a-5p. Methods Ox-LDL was used to induce human umbilical vein endothelial cells(HUVECs) to establish a cellular injury model. pcDNA-FGD5-AS1, anti-miR-106a-5p and its negative control were transfected into HUVECs and then added to ox-LDL-treated cells. pcDNA-FGD5-AS1 was co-transfected with miR-NC and miR-106a-5p mimics to HUVECs, then added ox-LDL to treat cells. RT-qPCR was used to detect the expression of FGD5-AS1 and miR-106a-5p. The level of MDA, SOD, and CAT was tested with commercially available kit. Flow cytometry was used to detect the apoptosis rate. The dual luciferase reporter gene experiment was used to detect the targeting relationship between FGD5-AS1 and miR-106a-5p. Western blot was used to detect the protein expression of cleaved caspase-3 and cleaved caspase-9. Results The expression of FGD5-AS1 mRNA in HUVECs induced by ox-LDL decreased (P＜0.05) while the expression of miR-106a-5p mRNA increased (P＜0.05). Both transfection of pcDNA-FGD5-AS1 and transfection of anti-miR-106a-5p reduced the level of MDA, apoptosis rate and protein level of cleaved caspase-3, cleaved caspase-9 in HUVECs induced by ox-LDL(P＜0.05),enhanced the activity of SOD and CAT (P＜0.05). FGD5-AS1 could target at miR-106a-5p. Co-transfection of pcDNA-FGD5-AS1 and miR-106a-5p mimics could reverse the effect of pcDNA-FGD5-AS1 on ox-LDL-induced HUVECs apoptosis and oxidative stress. Conclusions Over-expression of FGD5-AS1 may inhibit oxidative stress and apoptosis through targeted regulation of miR-106a-5p, thereby reducing ox-LDL-induced vascular endothelial cell damage.

Key words: lncRNA FGD5-AS1, miR-106a-5p, human umbilical vein endothelial cells, oxidative stress, apoptosis

CLC Number:

R394

GUO Jing, LI Ya-jie, MOU Han-shuang. LncRNA FGD5-AS1 reduces ox-LDL-induced damage of HUVECs by targeting miR-106a-5p[J]. Basic & Clinical Medicine, 2022, 42(5): 768-775.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/10.16352/j.issn.1001-6325.2022.05.026

http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2022/V42/I5/768

References

[1] 鲍金伟, 孙蓓, 马萍萍, 等. miRNA-27b靶向TET2基因对ox-LDL诱导内皮细胞炎症反应和细胞凋亡的影响[J]. 中华微生物学和免疫学杂志, 2019, 39: 856-863.
[2] 陈小兰, 陶福正, 郑道国, 等. GLP-1通过miR-22/NLRP3途径减轻ox-LDL诱导的内皮细胞焦亡[J]. 中国动脉硬化杂志, 2019, 27: 410-416.
[3] Bian W, Jing X, Yang Z, et al. Downregulation of lncRNA NORAD promotes ox-LDL-induced vascular endothelial cell injury and atherosclerosis[J]. Aging (Albany NY), 2020, 12: 6385-6400.
[4] Li H, Zhao Q, Chang L, et al. LncRNA MALAT1 modulates ox-LDL induced EndMT through the Wnt/beta-catenin signaling pathway[J]. Lipids Health Dis, 2019, 18: 62-72.
[5] Chen H, Lan Z, Li Q, et al. Abnormal expression of long noncoding RNA FGD5-AS1 affects the development of periodontitis through regulating miR-142-3p/SOCS6/NF-jB pathway[J]. Artif Cells Nanomed Biotechnol, 2019, 47: 2098-2106.
[6] Hu Y, Xu R, He Y, et al. Downregulation of microRNA-106a-5p alleviates ox-LDL-mediated endothelial cell injury by targeting STAT3[J]. Mol Med Rep, 2020, 22: 783-791.
[7] 朱为勇, 唐元升, 盖延红, 等. 和厚朴酚对ox-LDL诱导的血管内皮细胞损伤的保护作用及其机制研究[J]. 中国循证心血管医学杂志, 2018, 10: 1079-1083.
[8] Zheng Z, Zhang G, Liang X, et al. LncRNA OIP5-AS1 facilitates ox-LDL-induced endothelial cell injury through the miR-98-5p/HMGB1 axis[J]. Mol Cell Biochem, 2020, 29: 1-9.
[9] Cao L, Zhang Z, Li Y, et al. LncRNA H19/miR-let-7 axis participates in the regulation of ox-LDL-induced endothelial cell injury via targeting periostin[J]. Int Immunopharmacol, 2019, 72: 496-503.
[10] Xu X, Ma C, Duan Z, et al. LncRNA ZEB1-AS1 mediates oxidative low-density lipoprotein-mediated endothelial cells injury by post-transcriptional stabilization of NOD2[J]. Front Pharmacol, 2019, 10: 397-407.
[11] Guo JT, Wang L, Yu HB. Knockdown of NEAT1 mitigates ox-LDL-induced injury in human umbilical vein endothelial cells via miR-30c-5p/TCF7 axis[J]. Eur Rev Med Pharmacol Sci, 2020, 24: 9633-9644.
[12] Zhang XQ, Song LH, Feng SJ, et al. LncRNA FGD5-AS1 acts as a competing endogenous RNA for miRNA-223 to lessen oxygen-glucose deprivation and simulated reperfusion (OGD/R)-induced neurons injury[J]. Folia Neuropathol, 2019, 57: 357-365.
[13] Cai X, Zhang P, Wang S, et al. LncRNA FGD5 antisense RNA 1 upregulates RORA to suppress hypoxic injury of human cardiomyocyte cells by inhibiting oxidative stress and apoptosis via miR-195[J]. Mol Med Rep, 2020, 22: 4579-4588.
[14] 刘刚, 郭山岭, 胡永寸, 等. 沉默VCAM-1基因降低ox-LDL诱导的HUVECs氧化损伤和凋亡[J]. 基础医学与临床, 2019, 39: 1423-1426.
[15] 闫博阳, 赵津璋, 陈虹. PTEN在动脉粥样硬化中的表达及对血管内皮细胞增殖凋亡的影响研究[J]. 中国免疫学杂志, 2018, 34: 745-750.
[16] Ji Q, Qi D, Xu X, et al. Cryptotanshinone protects cartilage against developing osteoarthritis through the miR-106a-5p/GLIS3 axis[J]. Mol Ther Nucleic Acids, 2018, 11: 170-179.