CT120反义寡核苷酸抑制肺腺癌细胞A549的生长

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (4): 400-404.

CT120反义寡核苷酸抑制肺腺癌细胞A549的生长

李尊岭邵淑红谢书阳焦飞石寿森于雪艳

滨州医学院生物化学与分子生物学研究所滨州医学院滨州医学院滨州医学院山东大学医学院生物化学与分子生物学研究所

收稿日期:2008-07-10 修回日期:2008-09-02 出版日期:2009-04-25 发布日期:2009-04-25
通讯作者: 于雪艳

Antisense oligonecleotides of CT120 inhibited the growth of lung adenocarcinoma cell line A549

Zun-ling LI, Shu-hong SHAO, Shu-yang XIE, Fei JIAO, Shou-seng SHI, Xue-yan YU

Institute of Biochemistry and Molecular Biology, Binzhou Medical College Binzhou Medical College Binzhou Medical College Binzhou Medical College Institute of Biochemistry and Molecular Biology, School of Medicine, Shandong University

Received:2008-07-10 Revised:2008-09-02 Online:2009-04-25 Published:2009-04-25
Contact: Xue-yan YU

摘要/Abstract

摘要： 目的探讨CT120基因与肺腺癌细胞A549生长、分化的关系。方法构建含有CT120基因的反义表达载体（命名为pcDNA3.1-CT120），并将该载体转染肺腺癌细胞A549，应用G418筛选，获得了稳定表达CT120反义基因的克隆（命名为pcDNA3.1-CT120-A549）。RT-PCR和Western blot 检测CT120表达。流式细胞仪和软琼脂集落形成实验分析细胞生长，同时用RT-PCR检测P53、CYCLIND1 和CDK4的表达。结果成功构建了含有CT120基因的反义表达载体，而且该载体能够有效抑制CT120在肺腺癌细胞A549中的表达。抑制CT120基因的表达能够抑制细胞的生长，促进细胞凋亡。在稳定表达CT120反义基因的克隆中P53表达明显上调，CYCLIND1 和CDK4表达明显下调。结论反义寡核苷酸抑制CT120的表达有可能是肺癌基因治疗的一个新的靶点。

关键词: CT120, 反义寡核苷酸, 肺癌

Abstract: Object To clarify the relationship between CT120, a novel human plasma membrane-associated gene, and prolifetation of lung adenocarcinoma cell line A549. Method Expression vector（pcDNA3.1）containing antisense oligonucleotides of CT120 was constructed and transfected into the lung adenocarcinoma cell line A549. RT-PCR and Western blot detected the expression of CT120. Meantime, flow cytometry and soft agarose colony formation analyzed the cell proliferation, and P53、CYCLIND1 and CDK4 were detected by RT-PCR. Result pcDNA3.1 containing antisense oligonucleotides of CT120 was constructed successfully and could inhibit the expression of CT120 effectively by RT-PCR and Western blot. The expression of P53 was up-regulated and the expression of CYCLIND1、CDK4 were down-regulated. Conclusion The down-regulation of CT120 expression by antisense oligonucleotides technique may be a new candidate of drug target for treatment of lung cancer.

Key words: CT120, Antisense oligonecleotides, lung cancer

中图分类号:

R730.54

李尊岭邵淑红谢书阳焦飞石寿森于雪艳. CT120反义寡核苷酸抑制肺腺癌细胞A549的生长[J]. 基础医学与临床, 2009, 29(4): 400-404.

Zun-ling LI; Shu-hong SHAO; Shu-yang XIE; Fei JIAO; Shou-seng SHI; Xue-yan YU. Antisense oligonecleotides of CT120 inhibited the growth of lung adenocarcinoma cell line A549[J]. Basic & Clinical Medicine, 2009, 29(4): 400-404.

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