MEF2C调控DOK5的基因表达

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (4): 342-346.

MEF2C调控DOK5的基因表达

温见燕阴彬张英姿叶建伟廖文强于长安柯元南

中日友好医院中国医学科学院基础医学研究所北京协和医学院基础学院医学分子生物学国家重点实验室中国医学科学院基础医学研究所中日友好医院中日友好医院中日友好医院

收稿日期:2008-12-29 修回日期:2009-01-31 出版日期:2009-04-25 发布日期:2009-04-25
通讯作者: 柯元南

MEF2C regulates DOK5 gene expression

Jian-yan WEN, Ying-zi ZHANG, Jian-wei YE, Wen-qiang LIAO, Chang-an YU, Yuan-nan KE

China-Japan Friendship Hospital National Laboratory of Medicine Molecular Biology, IBMS, CAMS，School of Basic Medicine PUMC China-Japan Friendship Hospital

Received:2008-12-29 Revised:2009-01-31 Online:2009-04-25 Published:2009-04-25
Contact: Yuan-nan KE

摘要/Abstract

摘要： 目的研究MEF2C对DOK5的表达调控机制。方法用RT-PCR法检测小鼠中DOK5组织表达谱，检测P19CL6向心肌细胞分化过程中DOK5和MEF2C的表达。MEF2C与DOK5启动子报告基因共转染P19CL6细胞或将DOK5启动子上的MEF2结合位点突变后，观察报告基因活性的变化。结果DOK5在小鼠的脑、心脏、骨骼肌等高表达。DOK5和MEF2C在P19CL6向心肌细胞分化过程中mRNA表达水平升高 (p<0.05)。过表达MEF2C可以激活DOK5启动子区的报告基因活性，而MEF2结合位点突变则抑制DOK5启动子区报告基因活性。结论 MEF2C可以通过作用于DOK5启动子区的MEF2结合位点，调节DOK5的转录活性。

关键词: MEF2C, Dok5, 启动子, 基因表达调控

Abstract: Objective To explore the effects of myocyte enhancer factor 2C (MEF2C) on regulation of DOK5 during cardiomyocyte differentiation. Methods RT-PCR was carried out to detect the tissue distribution of mouse DOK5 gene and the expression level of DOK5 and MEF2C during P19CL6 cells differentiated into cardiomyocytes. The effect of MEF2C on DOK5 promoter was confirmed by co-transfection studies. Results The expression of DOK5 was rich in brain, muscle and heart. The mRNA level of DOK5 and MEF2C was enhanced during P19CL6 cells differentiation into cardiomyocytes (p<0.05). Over-expression of MEF2C could increase the promoter activity of DOK5. Furthermore, site-directed mutagenesis demonstrated that MEF2C was crucial for activating DOK5 promoter. Conclusion MEF2C enhanced the transcription of DOK5 through the MEF2 binding site on DOK5 promoter.

Key words: MEF2C, Dok5, promoter, regulation of gene expression

温见燕阴彬张英姿叶建伟廖文强于长安柯元南. MEF2C调控DOK5的基因表达[J]. 基础医学与临床, 2009, 29(4): 342-346.

Jian-yan WEN; Ying-zi ZHANG; Jian-wei YE; Wen-qiang LIAO; Chang-an YU; Yuan-nan KE. MEF2C regulates DOK5 gene expression[J]. Basic & Clinical Medicine, 2009, 29(4): 342-346.

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