P27kip1 与出核因子CRM1在淋巴瘤细胞中的表达及相互关系

基础医学与临床 ›› 2008, Vol. 28 ›› Issue (8): 820-824.

P27kip1 与出核因子CRM1在淋巴瘤细胞中的表达及相互关系

王燏婵赵玥铭沈爱国张冬梅黄晓东陆建新何松程纯

江苏省南通大学启秀校区微生物与免疫学教研室江苏省南通大学启秀校区微生物与免疫学教研室江苏省南通大学启秀校区微生物与免疫学教研室江苏省南通大学启秀校区微生物与免疫学教研室江苏省南通大学启秀校区微生物与免疫学教研室江苏省南通大学启秀校区微生物与免疫学教研室江苏省南通市肿瘤医院病理科江苏省南通大学启秀校区微生物与免疫学教研室

收稿日期:2007-04-25 修回日期:2007-12-25 出版日期:2008-08-25 发布日期:2008-08-25
通讯作者: 程纯

Expression and relationship of P27kip1 and nuclear export factor CRM1 in Lymphoma cell

Yu-chan WANG, Yue-ming ZHAO, AI-guo SHEN, Dong-mei ZHANG, Xiao-dong HUANG, Jian-xin LU, Song HE, Chun CHENG

Nantong University Department of Microbiology and Immunology, Nantong University Nantong University

Received:2007-04-25 Revised:2007-12-25 Online:2008-08-25 Published:2008-08-25
Contact: Chun CHENG

摘要/Abstract

摘要： 摘要目的：研究P27kip1与出核因子CRM1在淋巴瘤细胞中的表达情况及相互关系。方法：运用血清饥饿同步化处理淋巴瘤细胞株Jurkat和Raji，10%血清刺激细胞增殖。Western blot分别检测生长阻滞及增殖的淋巴瘤细胞中P27kip1与CRM1的表达情况。核浆分离方法检测P27kip1与CRM1分别在胞核、胞浆的定位。免疫沉淀检测P27kip1与CRM1在淋巴瘤细胞中的结合情况。结果：在不同状态的淋巴瘤细胞中，P27kip1与CRM1蛋白水平的表达均表现为负相关，并且P27kip1的核内外分布与CRM1的核内外分布一致。免疫沉淀结果验证在淋巴瘤细胞中P27kip1与CRM1相互结合。结论：出核因子CRM1可能通过与P27kip1结合而影响P27kip1的核内外分布及表达水平，从而参与调控淋巴瘤细胞的生长。

关键词: P27kip1, CRM1, 出核, 淋巴瘤

Abstract: Abstract: Objective To investigate the expression and relationship of P27kip1 and nuclear export factor CRM1 in lymphoma cells. Methods Cells were synchronized using serum starvation, 10%serum stimulating proliferation. The expression of P27kip1 and Jab1 were detected by cell fractionation and Western blot. LMB were used for stimulating Jurkat cells those were in proliferation process, and then detect the expression of P27kip1 and Jab1. Using immunoprecipitation detect the combination of P27kip1 and CRM1. Results The albumen amount of P27kip1 was always contrary with the albumen amount of CRM1. What’s more, the location of P27kip1 accorded with the expression of CRM1. P27kip1 and CRM1 could form compound in Jurkat and Raji cells detected by immunoprecipitation. Conclusion These indicated the CRM1 may influence the location and expression of P27kip1 through integrating with P27kip1, and then may participate in regulating the growth of NHL cells.

Key words: P27kip1, CRM1, export, Lymphoma

王燏婵赵玥铭沈爱国张冬梅黄晓东陆建新何松程纯. P27kip1 与出核因子CRM1在淋巴瘤细胞中的表达及相互关系[J]. 基础医学与临床, 2008, 28(8): 820-824.

Yu-chan WANG; Yue-ming ZHAO; AI-guo SHEN; Dong-mei ZHANG; Xiao-dong HUANG; Jian-xin LU; Song HE; Chun CHENG. Expression and relationship of P27kip1 and nuclear export factor CRM1 in Lymphoma cell[J]. Basic & Clinical Medicine, 2008, 28(8): 820-824.

[1]	杨华夏郭静波肖欣悦张烜. 多发性肌炎/皮肌炎合并淋巴瘤的临床特点分析[J]. 基础医学与临床, 2017, 37(2): 234-237.
[2]	唐敏武晓楠程刚. Crizotinib在ALK基因阳性非小细胞肺癌中的临床研究进展[J]. 基础医学与临床, 2013, 33(3): 382-386.
[3]	鲁云彪陈厚早张冉郑伟李莉张庆军刘德培. SIRT1上调大鼠血管平滑肌细胞p27Kip1的表达[J]. 基础医学与临床, 2010, 30(7): 714-720.
[4]	朱维培张弘钱志红刘利芬. 卵巢癌细胞株中c-Jun激活蛋白-1与p27kip1表达的相关性[J]. 基础医学与临床, 2009, 29(9): 955-960.
[5]	杨拥军师杰崔全才. 皮下脂膜炎样T细胞淋巴瘤6例临床病理分析[J]. 基础医学与临床, 2009, 29(5): 542-546.
[6]	白向阳严玲玲朱涛. 淋巴组织中新基因C14orf44的生物信息学分析[J]. 基础医学与临床, 2007, 27(10): 1113-1117.