狼疮肾炎小鼠肾脏的比较蛋白质组学研究

基础医学与临床 ›› 2008, Vol. 28 ›› Issue (3): 256-259.

狼疮肾炎小鼠肾脏的比较蛋白质组学研究

施孟如林全吕建新

温州医学院生物学实验教学中心温州医学院生物学实验教学中心温州医学院生物学实验教学中心

收稿日期:2007-06-11 修回日期:2007-07-10 出版日期:2008-03-25 发布日期:2008-03-25
通讯作者: 施孟如

Comparative proteomics study of the kidney in lupus nephritis mouse

Meng-ru SHI, Quan LIN, Jian-xin LV

Central Laboratory of Biology, Wenzhou Medical College

Received:2007-06-11 Revised:2007-07-10 Online:2008-03-25 Published:2008-03-25
Contact: Meng-ru SHI,

摘要/Abstract

摘要： 目的探讨狼疮肾炎(LN)小鼠肾脏和正常小鼠肾脏蛋白质组的差异。方法应用双向凝胶电泳技术(two-dimensional gel electrophoresis,2-DE)分离肾组织总蛋白质，凝胶用银染显色，扫描仪获取凝胶图像并对其进行软件分析。结果同组实验重复3次，其中正常对照组3张图找到蛋白质点平均573±52个，匹配率为83％；LN组找到蛋白质点平均658±43个, 匹配率为87％。经软件分析，LN组和对照组比较表达增加大于2倍的点有119个；降低大于0.5倍的点有140个。结论 LN小鼠肾脏蛋白质表达发生了显著变化，这可能和LN的发生发展有着密切关系。

关键词: 狼疮肾炎, 系统性红斑狼疮, 肾脏, 蛋白质组, 双向电泳

Abstract: Objective To study the differential proteome of kidney between lupus nephritis mouse and normal mouse. Methods The proteins of kidney were separated by two-dimensional gel electrophoresis(2-DE). The gels stained by silver were scanned by ImageScanner and analyzed by PDQuest software. Results The experiments were repeated for three times. About 573±52 and 658±43 protein spots were found in the three maps of control group and LN group respectively; the match ratio is 83% and 87% respectively. Analyzed by PDQuest software,114 spots were found increased for two folds through comparing LN group to control group,and 140 spots were found decrease for 0.5 folds. Conclusions A significant differences in protein expression of LN mouse kidney,which may be related to the etiopathogenesis of LN,had been found.

Key words: lupus nephritis, systemic lupus erythematosus, kidney, proteome, two dimensional electrophoresis

施孟如林全吕建新. 狼疮肾炎小鼠肾脏的比较蛋白质组学研究[J]. 基础医学与临床, 2008, 28(3): 256-259.

Meng-ru SHI; Quan LIN; Jian-xin LV. Comparative proteomics study of the kidney in lupus nephritis mouse[J]. Basic & Clinical Medicine, 2008, 28(3): 256-259.

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